3L7H
Insights into dynein assembly from a dynein intermediate chain light chain Roadblock structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 133.15 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.218, 58.693, 65.095 |
Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
Resolution | 63.720 - 1.950 |
R-factor | 0.19938 |
Rwork | 0.193 |
R-free | 0.25417 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hz5 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.931 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.720 | 2.001 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.139 | 0.092 |
Number of reflections | 27041 | |
<I/σ(I)> | 2.5 | 5.5 |
Completeness [%] | 98.9 | 94.7 |
Redundancy | 4.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277.15 | 20% iso-propanol, 20% PEG 4000, 100 mM sodium citrate., pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |