3L7E

Crystal structure of ANTI-IL-13 antibody C836

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	CCD
Collection date	2007-06-11
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	97.940, 141.460, 76.580
Unit cell angles	90.00, 117.46, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.20379
Rwork	0.201
R-free	0.25075
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l5w
RMSD bond length	0.009
RMSD bond angle	1.320
Data reduction software	d*TREK (9.6L)
Data scaling software	d*TREK (9.6L)
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.408
Number of reflections	30327
<I/σ(I)>	10.4	3
Completeness [%]	94.8	75.7
Redundancy	7.7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M SODIUM ACETATE PH 4.5, 0.2 M AMMONIUM SULFATE, 16% PEG 4K; CRYO CONDITIONS: SODIUM ACETATE PH 4.5, 0.2 M AMMONIUM SULFATE, 20% PEG 4K, 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K