3L7D
Crystal Structure of Glycogen Phosphorylase DK5 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.132, 129.132, 117.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.270 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.493 |
| Number of reflections | 65604 | |
| <I/σ(I)> | 4.9 | 2.2 |
| Completeness [%] | 97.5 | 98.3 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10MM BES BUFFER, 3MM DDT, pH 6.7, small tubes, temperature 289K |
