3L7C
Crystal Structure of Glycogen Phosphorylase DK4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.096, 129.096, 116.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.930 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 74225 | |
| <I/σ(I)> | 20.8 | 4.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10MM BES BUFFER, 3MM DDT, pH 6.7, small tubes, temperature 289K |
