3L72
Chicken cytochrome BC1 complex with kresoxim-I-dimethyl bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 172.614, 181.548, 241.033 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.930 - 3.060 |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT USING NATIVE STRUCTURE SOLVED BY THE SAME AUTHOR |
Starting model (for MR) | 1bcc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 141091 | |
<I/σ(I)> | 8.48 | 0.656 |
Completeness [%] | 93.2 | 85.7 |
Redundancy | 3.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 20MM KMES PH 6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |