3L6W
Structure of the collar functional unit (KLH1-H) of keyhole limpet hemocyanin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-09-22 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 21 3 |
Unit cell lengths | 251.020, 251.020, 251.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 4.000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1js8 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.200 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.180 | 0.847 |
Number of reflections | 22294 | |
<I/σ(I)> | 10.6 | 3.46 |
Completeness [%] | 96.8 | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 9% PEG 1000, 50mM phosphate/citrate buffer, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |