3L6T
Crystal Structure of an N-terminal Mutant of the Plasmid pCU1 TraI Relaxase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.385, 58.656, 86.532 |
| Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
| Resolution | 31.374 - 1.930 |
| R-factor | 0.1694 |
| Rwork | 0.168 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omh Plasmid R388 TrwC Relaxase Domain |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 36385 | |
| <I/σ(I)> | 31.589 | 3.03 |
| Completeness [%] | 95.5 | 76 |
| Redundancy | 7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 250mM triNa citrate, 22% PEG 3350, 5mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
