3L6O
Crystal Structure of Phosphate bound apo Glyceraldehyde-3-phosphate dehydrogenase 1 from MRSA252 at 2.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.955, 93.683, 89.054 |
| Unit cell angles | 90.00, 106.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H48 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.469 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.8L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.340 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.088 | 0.428 |
| Total number of observations | 17409 | |
| Number of reflections | 53348 | |
| <I/σ(I)> | 6.8 | 2.3 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 3.61 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 298 | 0.1M Tris-HCl pH 8.7, 32% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
