3L64
T4 Lysozyme S44E/WT*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | Xuong-Hamlin |
| Collection date | 1993 |
| Detector | Multiwire Area Detector |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.100, 35.100, 46.800 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.400 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.900 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.047 | |
| Number of reflections | 13554 | |
| Completeness [%] | 91.0 | 65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 Ul of protein solution (20 mg/ml) mixed with 10 Ul of well solution, typically 2.0 M-phosphate (pH 6.3 to 7.1), 0.25 M-NaCl and 6 Ul of 2-hydroxyethyl disulfide/ml (i.e. oxidized beta-mercaptoethanol). The drop was then equilibrated over 1 ml of well solution using a Limbro plate, VAPOR DIFFUSION, HANGING DROP |
