3L5X

Crystal structure of the complex between IL-13 and H2L6 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	63.780, 73.020, 114.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.20903
Rwork	0.197
R-free	0.24679
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1Q9Q
RMSD bond length	0.012
RMSD bond angle	1.387
Data reduction software	d*TREK (9.6L)
Data scaling software	d*TREK (9.6L)
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.084	0.392
Number of reflections	42621
<I/σ(I)>	14.5	2.3
Completeness [%]	98.9	91.1
Redundancy	16.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES PH 6.5, 14% PEG 3350, 0.2 M AMMONIUM TARTRATE. CRYO CONDITIONS: 0.1 M MES PH 6.5, 20% PEG 3K, 0.2 M AMM TARTRATE, 15% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K