3L5J
Crystal structure of FnIII domains of human GP130 (Domains 4-6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95443 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.936, 89.221, 106.877 |
| Unit cell angles | 90.00, 117.72, 90.00 |
Refinement procedure
| Resolution | 35.154 - 3.042 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.621 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 2.650 | 3.050 |
| Number of reflections | 16494 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, NaCl, Tris-HCl, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
