3L4B
Crystal Structure of an Octomeric Two-Subunit TrkA K+ Channel Ring Gating Assembly, TM1088A:TM1088B, from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.778, 96.778, 305.098 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.390 - 3.450 |
R-factor | 0.26134 |
Rwork | 0.259 |
R-free | 0.30827 |
Structure solution method | MAD |
Starting model (for MR) | 2g1u |
RMSD bond length | 0.006 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.390 | 3.570 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 22866 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Magnesium Chloride, 15% PEG 4000, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |