3L4A
Crystal Structure of an Anopheles gambiae Odorant-binding Protein 22a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-22 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.194, 37.729, 43.703 |
Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
Resolution | 25.465 - 1.500 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l47 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.846 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.036 | 0.026 | 0.181 |
Number of reflections | 17623 | ||
<I/σ(I)> | 38.2 | ||
Completeness [%] | 94.0 | 99.6 | 82.8 |
Redundancy | 4.2 | 6.9 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium citrate tribasic, MPD, pH 7.0, vapor diffusion, hanging drop, temperature 298K |