3L48

Crystal structure of the C-terminal domain of the PapC usher

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-19
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	100.936, 100.936, 89.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.140 - 2.100
R-factor	0.2011
Rwork	0.200
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_241))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.140
High resolution limit [Å]	2.100
Number of reflections	27641
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% Tacsimate pH 8.0, Cryoprotected in 60% Tacsimate and 10% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 293K