3L40
Crystal Structure of S. pombe Brc1 BRCT5-BRCT6 domains
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.111, 0.9796, 0.9797, 1.0087 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.472, 83.425, 87.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.785 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 61324 | |
| <I/σ(I)> | 38.6 | 2.2 |
| Completeness [%] | 94.8 | 67 |
| Redundancy | 6.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 295 | 20-22% w/v PEG 3350, 100 mM NaCl, 100 mM K2HPO4/KH2PO4 pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
