3L3Q
Mouse importin alpha-pepTM NLS peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.55 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.756, 90.899, 100.968 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.790 - 2.300 |
| R-factor | 0.18891 |
| Rwork | 0.187 |
| R-free | 0.22662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pjn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.921 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.094 | 0.094 |
| Number of reflections | 29414 | |
| Completeness [%] | 89.6 | 89.3 |
| Redundancy | 3.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Sodium Citrate, DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
