3L3F

Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789, 0.9792, 0.9640
Spacegroup name	P 61
Unit cell lengths	103.115, 103.115, 72.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.426 - 1.900
R-factor	0.19594
Rwork	0.195
R-free	0.21877
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.750	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.256
Number of reflections	34591
Completeness [%]	100.0	100
Redundancy	11.2	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Tris-HCl (pH 7.5), 28% PEG 3350, 0.1M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K