3L3B
Crystal structure of isoprenoid biosynthesis protein with amidotransferase-like domain from Ehrlichia Chaffeensis at 1.90A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.210, 69.220, 242.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.560 - 1.900 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb deposition 1vhq modified with CCP4 program CHAINSAW |
RMSD bond length | 0.017 |
RMSD bond angle | 1.528 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.465 |
Number of reflections | 32534 | |
<I/σ(I)> | 20.59 | 4.4 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.3 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | EBS JCSG+ SCREEN G10: 150mM Postassium bromice, 30% PEG 2000 MME; EHCHA.01061.A AT 20MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |