3L2K
Structure of phenazine antibiotic biosynthesis protein with substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2008-09-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 3 |
Unit cell lengths | 193.129, 193.129, 193.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.780 - 2.800 |
R-factor | 0.21635 |
Rwork | 0.212 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hgu |
RMSD bond length | 0.030 |
RMSD bond angle | 2.713 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.280 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | |
Number of reflections | 29538 | |
<I/σ(I)> | 12 | 3.2 |
Redundancy | 14.7 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Na/K Phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |