3L2A
Crystal structure of Reston Ebola VP35 interferon inhibitory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 51.551, 51.551, 50.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.990 - 1.710 |
| R-factor | 0.17257 |
| Rwork | 0.170 |
| R-free | 0.22528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fke |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.700 | 0.603 |
| Number of reflections | 13734 | |
| <I/σ(I)> | 39 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 293 | 16% PEG3350, 0.1M SODIUM ACETATE, 14% TACSIMATE, pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
