3L1O
Crystal structure of monoclonal antibody MN423 Fab fragment with free combining site, crystallized in the presence of zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8166 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.330, 75.426, 72.701 |
Unit cell angles | 90.00, 92.86, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.000 |
R-factor | 0.16237 |
Rwork | 0.158 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v17 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.192 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.415 |
Number of reflections | 24684 | |
<I/σ(I)> | 5 | |
Completeness [%] | 84.6 | 99.7 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 10% PEG 3350, 0.1M IMIDAZOLE, 0.01M ZNSO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |