3L1N
Crystal structure of Mp1p ligand binding domain 2 complexd with palmitic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97894 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.510, 46.510, 149.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.740 - 1.300 |
R-factor | 0.18242 |
Rwork | 0.182 |
R-free | 0.19755 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.043 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.740 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.079 | 0.383 |
Number of reflections | 40950 | |
<I/σ(I)> | 5.5 | |
Completeness [%] | 98.0 | 95 |
Redundancy | 20.2 | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 11% polyethylene glycol(PEG)8000, 0.1M HEPES(pH 7.5), 8% ethylene glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |