3L1L
Structure of Arg-bound Escherichia coli AdiC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 84.690, 127.170, 157.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.296 - 3.002 |
R-factor | 0.2244 |
Rwork | 0.222 |
R-free | 0.27870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3H5M |
RMSD bond length | 0.027 |
RMSD bond angle | 1.646 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.296 | 3.110 |
High resolution limit [Å] | 3.002 | 3.000 |
Rmerge | 0.068 | 0.961 |
Number of reflections | 17222 | |
<I/σ(I)> | 25.8 | 2 |
Completeness [%] | 99.2 | 99.8 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 0.1mM Hepes pH 7.5, 26% (w/v) PEG 600 with 0.05% DSM as additive, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |