3L1F
Bovine AlphaA crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 56.059, 56.059, 69.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.462 - 1.530 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.050 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 3.300 | 1.530 |
| Rmerge | 0.041 | 0.023 | 0.496 |
| Number of reflections | 16465 | ||
| <I/σ(I)> | 16.6 | ||
| Completeness [%] | 94.9 | 82.6 | 98.6 |
| Redundancy | 6.8 | 5.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 10% PEG 6000, 5% MPD, vapor diffusion, sitting drop, temperature 298K |
