3L19
Crystal structure of calcium binding domain of CpCDPK3, cgd5_820
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.570, 64.187, 72.807 |
| Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
| Resolution | 19.350 - 2.140 |
| Rwork | 0.215 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aao |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.069 | 0.595 |
| Number of reflections | 22779 | |
| <I/σ(I)> | 17.88 | 2.99 |
| Completeness [%] | 97.5 | 85.8 |
| Redundancy | 7.39 | 6.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30% PEG550MME, 0.05M Magnesium Chloride, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
