3L10
Structure of split monoubiquitinated PCNA with ubiquitin in position one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-25 |
Detector | NOIR-1 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 3 |
Unit cell lengths | 122.516, 122.516, 122.516 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.000 - 2.800 |
R-factor | 0.279 |
Rwork | 0.277 |
R-free | 0.31400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
RMSD bond length | 0.017 |
RMSD bond angle | 1.587 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.630 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.109 | 0.604 |
Number of reflections | 15333 | |
<I/σ(I)> | 10.5 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.74 | 5.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 2.04M ammonium sulfate, 0.1M sodium citrate, 3% ethanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |