3L0I
Complex structure of SidM/DrrA with the wild type Rab1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.808, 71.747, 146.911 |
| Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
| Resolution | 34.940 - 2.850 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.26600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.039 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.103 | 0.542 |
| Number of reflections | 30947 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1.1M ammonium sulfate, 0.1M sodium citrate, 0.4M Li2SO4, 0.1M di-ammonium tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
