3KZV
The crystal structure of a cytoplasmic protein with unknown function from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 117.413, 117.413, 89.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.050 - 2.000 |
R-factor | 0.19409 |
Rwork | 0.191 |
R-free | 0.24461 |
Structure solution method | SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 2.284 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.050 | 2.052 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.148 | 0.536 |
Number of reflections | 20048 | |
<I/σ(I)> | 1.5 | 36.9 |
Completeness [%] | 98.7 | 93.58 |
Redundancy | 16.3 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.1M Bis-tris, 5% Iso-propanol, 2.4M NH4SO4,1/1600 chymotrypsin, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |