3KZQ
The crystal structure of the protein with unknown function from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-06 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.304, 118.108, 206.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.782 - 2.100 |
| R-factor | 0.18132 |
| Rwork | 0.179 |
| R-free | 0.21836 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.149 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.540 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.121 | 0.560 |
| Number of reflections | 115617 | |
| <I/σ(I)> | 16.45 | 2.18 |
| Completeness [%] | 98.4 | 93.44 |
| Redundancy | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 20% PEG8000, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
