3KZQ
The crystal structure of the protein with unknown function from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-06 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.304, 118.108, 206.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.782 - 2.100 |
R-factor | 0.18132 |
Rwork | 0.179 |
R-free | 0.21836 |
Structure solution method | SAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.149 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.540 | 2.155 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.121 | 0.560 |
Number of reflections | 115617 | |
<I/σ(I)> | 16.45 | 2.18 |
Completeness [%] | 98.4 | 93.44 |
Redundancy | 7.4 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 20% PEG8000, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |