3KZI
Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.890, 224.690, 337.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 3.600 |
| R-factor | 0.297 |
| Rwork | 0.297 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BZ1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.112 | 0.477 |
| Number of reflections | 47417 | |
| <I/σ(I)> | 11.35 | 2.34 |
| Completeness [%] | 89.2 | 68.9 |
| Redundancy | 4.11 | 4.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7 | 291 | PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K |
