3KZG
Crystal structure of an arginine 3rd transport system periplasmic binding protein from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.965, 148.254, 149.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.310 - 2.060 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.310 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.130 | 0.236 |
Number of reflections | 58467 | |
<I/σ(I)> | 12.2 | 2.5 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 13 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG 3350, 0.1M Bis-Tris, 0.2M Lithium sulfate monohydrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |