3KZB
Crystal structure of xylulokinase from Chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-11-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.893, 159.799, 47.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.387 - 2.705 |
R-factor | 0.1926 |
Rwork | 0.189 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hz6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.168 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.064 | 0.150 |
Number of reflections | 15256 | |
<I/σ(I)> | 17.1 | 4.7 |
Completeness [%] | 97.9 | 100 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 0.1 M HEPES pH7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 110K |