3KZA
Crystal structure of Gyuba, a patched chimera of b-lactglobulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 277 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 65 |
Unit cell lengths | 68.925, 68.925, 151.616 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 59.660 - 2.000 |
R-factor | 0.23718 |
Rwork | 0.235 |
R-free | 0.28491 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.990 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.660 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 27566 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 10.9 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.0M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |