3KZA

Crystal structure of Gyuba, a patched chimera of b-lactglobulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	277
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 65
Unit cell lengths	68.925, 68.925, 151.616
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.660 - 2.000
R-factor	0.23718
Rwork	0.235
R-free	0.28491
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.990
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.660	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	27566
<I/σ(I)>	15.2
Completeness [%]	99.8	99.9
Redundancy	10.9	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.0M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K