3KZ3
A structure of a lambda repressor fragment mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-3 |
| Synchrotron site | SSRL |
| Beamline | BL9-3 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-09-26 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.630, 58.710, 42.850 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.640 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.067 | |
| Number of reflections | 19544 | |
| Completeness [%] | 99.1 | 90.9 |
| Redundancy | 7.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
