3KYZ
The crystal structure of the sensor domain of two-component sensor PfeS from Pseudomonas aeruginosa PA01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97932 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 29.920, 95.468, 103.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.497 |
R-factor | 0.1527 |
Rwork | 0.151 |
R-free | 0.18110 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.095 | 0.731 |
Number of reflections | 24347 | |
<I/σ(I)> | 41.4 | 2.5 |
Completeness [%] | 99.7 | 97.4 |
Redundancy | 8.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 297 | 0.2M MgCl2, 0.1M Bis-Tris, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |