3KYI

Crystal structure of the phosphorylated P1 domain of CheA3 in complex with CheY6 from R. sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.400
Spacegroup name	P 1
Unit cell lengths	33.170, 43.433, 48.647
Unit cell angles	78.81, 86.57, 80.87

Refinement procedure

Resolution	34.990 - 2.800
R-factor	0.243
Rwork	0.241
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyj
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.120	0.050	0.616
Number of reflections	6518
<I/σ(I)>	5.2
Completeness [%]	96.1	97.4	94.5
Redundancy	1.8	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	295	100mM Bicine, pH 9, 1M LiCl, 20% (w/v) PEG 6000, vapor diffusion, temperature 295K