3KYF

Crystal structure of P4397 complexed with c-di-GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	200
Detector technology	CCD
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	112.700, 41.304, 61.590
Unit cell angles	90.00, 92.21, 90.00

Refinement procedure

Resolution	33.010 - 2.100
R-factor	0.194
Rwork	0.194
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	15600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris-HCl, pH 8.5, 25% PEG 3350, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K