3KY9
Autoinhibited Vav1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-15 |
| Detector | SBC-3 |
| Wavelength(s) | 1.28317 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.376, 58.738, 160.721 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 47.286 - 2.731 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.27050 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.780 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.076 | 0.959 |
| Number of reflections | 42421 | |
| <I/σ(I)> | 44.8 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.3 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.02 M TRIS, 0.05 M NACL, 5% GLYCEROL, 2 mM TCEP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
