3KY7
2.35 Angstrom resolution crystal structure of a putative tRNA (guanine-7-)-methyltransferase (trmD) from Staphylococcus aureus subsp. aureus MRSA252
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 125.176, 125.176, 125.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.320 - 2.350 |
R-factor | 0.21079 |
Rwork | 0.209 |
R-free | 0.25535 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.746 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.052 | 0.542 |
Number of reflections | 14553 | |
<I/σ(I)> | 35.78 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 7.6 mg/mL protein mixed at 1:1 v/v with 1.4 M Na Malonate pH 7.0, 0.1 M Bis-Tris Propane, VAPOR DIFFUSION, SITTING DROP, temperature 295K |