3KXT

Crystal structure of Sulfolobus Cren7-dsDNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97960
Spacegroup name	C 2 2 21
Unit cell lengths	51.190, 53.115, 90.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.732 - 1.602
R-factor	0.178
Rwork	0.176
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES; 2JTM 1azp
RMSD bond length	0.010
RMSD bond angle	1.613
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.060	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	16134
<I/σ(I)>	37.262	2.7
Completeness [%]	97.2	83.8
Redundancy	9.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	1.0mM complex, 0.1M tri-sodium citrate dihydrate pH 5.6, 20% (v/v) iso-propanol, 20% (w/v) polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K