3KXC
Mutant transport protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.172, 69.265, 121.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.280 - 2.000 |
| R-factor | 0.19799 |
| Rwork | 0.196 |
| R-free | 0.23238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cfh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.115 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 26752 | |
| <I/σ(I)> | 16.8 | 3 |
| Completeness [%] | 94.9 | 87.6 |
| Redundancy | 4.05 | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 22% PEG 3350, 0.2M Li2SO4, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
