3KWE
Inactive truncation of the beta-carboxysomal gamma-Carbonic Anhydrase, CcmM, form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91840 |
Spacegroup name | H 3 |
Unit cell lengths | 102.931, 102.931, 44.903 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.950 - 1.100 |
R-factor | 0.12944 |
Rwork | 0.129 |
R-free | 0.13947 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kwd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.411 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.066 | 0.294 |
Number of reflections | 65832 | |
<I/σ(I)> | 9.7 | 4.1 |
Completeness [%] | 91.4 | 64.2 |
Redundancy | 5.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.01M cobalt (II) chloride, 0.1M sodium acetate, 1.0 M 1,6-hexanediol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |