3KWD
Inactive truncation of the beta-carboxysomal gamma-Carbonic Anhydrase, CcmM, form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91840 |
Spacegroup name | H 3 |
Unit cell lengths | 103.006, 103.006, 44.953 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.970 - 1.100 |
R-factor | 0.13344 |
Rwork | 0.133 |
R-free | 0.14769 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qre |
RMSD bond length | 0.008 |
RMSD bond angle | 1.383 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 70535 | |
<I/σ(I)> | 24.1 | 2.66 |
Completeness [%] | 97.8 | 77.2 |
Redundancy | 3.25 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 14 mg/ml protein, 1.0 M ammonium sulphate, 0.1 M Bis-Tris, 1% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |