3KWC
Oxidized, active structure of the beta-carboxysomal gamma-Carbonic Anhydrase, CcmM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.900, 105.040, 196.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.880 - 2.000 |
| R-factor | 0.20217 |
| Rwork | 0.200 |
| R-free | 0.24702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kwd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 89407 | |
| <I/σ(I)> | 16.6 | 4.6 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 7.45 | 6.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 58 mg/ml protein, 20% isopropanol, 30% PEG 4000, 0.1 M Na Citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
