3KW7
Crystal structure of LacB from Trametes sp. AH28-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 98.778, 98.778, 149.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.440 |
| R-factor | 0.22634 |
| Rwork | 0.223 |
| R-free | 0.29165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gyc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.504 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.800 | 3.550 |
| High resolution limit [Å] | 3.400 | 3.440 |
| Number of reflections | 17991 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 283 | 0.1M Sodium citrate tribasic dihydrate (pH 5.2), 25% PEG 4000, 10% iso-propanol , VAPOR DIFFUSION, HANGING DROP, temperature 283K |
