3KVG

Crystal structure of the N-terminal domain of Hsp70 (cgd2_20) from Cryptosporidium parvum in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2009-11-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	86.644, 115.449, 179.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.150
Rwork	0.183
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.110
Number of reflections	49144
<I/σ(I)>	8.1
Completeness [%]	100.0	100
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	292	10% PEG 4000, 0.1 M NaAcetate, 0.1 M NaAcetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K