3KVE
Structure of native L-amino acid oxidase from Vipera ammodytes ammodytes: stabilization of the quaternary structure by divalent ions and structural changes in the dynamic active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.978, 95.839, 108.442 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 27.400 - 2.570 |
| R-factor | 0.19537 |
| Rwork | 0.191 |
| R-free | 0.27609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.523 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.172 | 0.448 |
| Number of reflections | 62717 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.1 | 74.7 |
| Redundancy | 7.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
