3KVD
Crystal structure of the Neisseria meningitidis Factor H binding protein, fHbp (GNA1870) at 2.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.2 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.097, 83.097, 71.301 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.000 - 2.000 |
R-factor | 0.22844 |
Rwork | 0.227 |
R-free | 0.24988 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w80 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.541 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.220 | 0.470 |
Number of reflections | 17912 | |
<I/σ(I)> | 6.6 | 1.8 |
Completeness [%] | 98.0 | 90.7 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 25% PEG 2000 MME, 300 mM Na acetate, 100 mM Tris, pH 7.5, VAPOR DIFFUSION, temperature 293K |