3KV6
Structure of KIAA1718, human Jumonji demethylase, in complex with alpha-ketoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.400, 125.200, 206.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.540 - 2.890 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2YU2 AND 1WEP |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.540 | 2.990 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.119 | 0.557 |
Number of reflections | 70224 | |
<I/σ(I)> | 16.5 | 4 |
Completeness [%] | 97.6 | 95.6 |
Redundancy | 7.8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 5-10% (v/v) polyethylene glycol 3350, 0.2 M KSCN, and 0.1 M BisTris pH 6.0, VAPOR DIFFUSION, HANGING DROP |