3KV5
Structure of KIAA1718, human Jumonji demethylase, in complex with N-oxalylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.700, 125.600, 206.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.820 - 2.390 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2YU2 AND 1WEP |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.820 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.055 | 0.637 |
| Number of reflections | 62010 | |
| <I/σ(I)> | 9.7 | 1.7 |
| Completeness [%] | 94.8 | 81.3 |
| Redundancy | 8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 5-10% (v/v) polyethylene glycol 3350, 0.2 M KSCN, and 0.1 M BisTris pH 6.0, VAPOR DIFFUSION, HANGING DROP |
